Detection of polymyxin B resistance in Enterobacterales by broth disk elution method

Background: Antibiotic resistance being the critical issue faced by medical field, especially Gram negative bacteria. It is a great threat in case of both hospitals acquired and community infections. They possess various mechanisms for their survival. The widespread bacteria has necessitated evaluation use older antimicrobials such as polymyxins. Polymyxins are disfavoured owing to potential clinical toxicity, nephrotoxicity. Thus, they got abandoned sixties. But now re- emerged used last resort antibiotics. Methods: 274 isolates Enterobacterales including Klebsiella pneumoniae, Escherichia coli, Citrobacter spp. Enterobacter was collected from diagnostic microbiology laboratories Kerala. polymyxin among broth disk elution method recommended CLSI. Results: In this study prevalence multi drug resistant 37% extensively strains 25%. And threatening fact that colistin also shows (9.2%). Conclusions: Though Polymyxin B lesser side present study, increase agent documented globally elsewhere. So, rational warrantied we could cherish “life - saving drug” avoid no available. Knowledge antimicrobial development new agents improved treatments essential current situation.